Parallel Profiling of DNA and Transient Epigenomic

Parallel Profiling of DNA and Transient Epigenomic

Table of Contents

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Instructions

Parallel Profiling of DNA and Transient Epigenomic

For this forum, you will critique 2 classmate’s poster presentations. In your thread, submit your

poster presentation (PDF format is preferred).

Then, review 2 classmate’s posters, identifying at least 2 strengths and 2 weaknesses in the

poster presentation and give possible suggestions on how the poster might be improved (each

reply must be at least 250 words). These can be based on, but not limited to, the following

categories:

Flow and alignment of the poster elements,

Choice of color and font,

Format of images and figures, and

Vocabulary and definitions.

Parallel Profiling of DNA and Transient Epigenomic

Hypothesis Hypoxia stimulates EMT and Metastasis Questions

We assume that HIF is the main molecule responsible for this outcome (12 points).

3. For each of the three experiments:

d) List the methods you used to conduct the experiment (sometimes it will be several, like cell

transfection, followed by immunoprecipitation, and western blot). Remember to show the

same experimental outcome with two different methods or with pharmacologic (drug) and

genetic (siRNA or CRISPR or gene overexpression) ways to alter protein activity/expression. You

Parallel Profiling of DNA and Transient Epigenomic

can cluster those under the same “experiment.” Prioritize “cause and consequence”

experiments over “correlative”. Make one of the three experiments in vivo (in animals).

a) List your experimental groups, including the controls (what do you compare to what? No

explanation needed). If you are going to use chemical treatments, indicate tentative

concentrations and treatment time. If you are using antibodies, state what they recognize.

b) What are the expected results if the hypothesis is right, e.g. after protein X was knocked

down/overexpressed/inhibited, metastasis

Parallel Profiling of DNA and Transient Epigenomic

RUBRIC
Excellent Quality 95-100%	Introduction 45-41 points The background and significance of the problem and a clear statement of the research purpose is provided. The search history is mentioned.		Literature Support 91-84  points The background and significance of the problem and a clear statement of the research purpose is provided. The search history is mentioned.			Methodology 58-53 points Content is well-organized with headings for each slide and bulleted lists to group related material as needed. Use of font, color, graphics, effects, etc. to enhance readability and presentation content is excellent. Length requirements of 10 slides/pages or less is met.
Average Score 50-85%	40-38 points More depth/detail for the background and significance is needed, or the research detail is not clear. No search history information is provided.		83-76  points Review of relevant theoretical literature is evident, but there is little integration of studies into concepts related to problem. Review is partially focused and organized. Supporting and opposing research are included. Summary of information presented is included. Conclusion may not contain a biblical integration.			52-49  points Content is somewhat organized, but no structure is apparent. The use of font, color, graphics, effects, etc. is occasionally detracting to the presentation content. Length requirements may not be met.
Poor Quality 0-45%	37-1 points The background and/or significance are missing. No search history information is provided.		75-1 points Review of relevant theoretical literature is evident, but there is no integration of studies into concepts related to problem. Review is partially focused and organized. Supporting and opposing research are not included in the summary of information presented. Conclusion does not contain a biblical integration.			48-1 points There is no clear or logical organizational structure. No logical sequence is apparent. The use of font, color, graphics, effects etc. is often detracting to the presentation content. Length requirements may not be met
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