Borough of Manhattan Bacterial Mutagenesis Mutation Discussion

Borough of Manhattan Bacterial Mutagenesis Mutation Discussion

Table of Contents

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Instructions

Borough of Manhattan Bacterial Mutagenesis Mutation Discussion

The lab report will consist of 2 pages: one page for tables and figures, one page for the

Discussion. The majority of your discussion should give MEANING to the results.  That

is, the discussion doesn't just restate the results; it explains what the results mean for

the experiment, for the lab, and for genetics and biology in general.

Guidelines: Borough of Manhattan Bacterial Mutagenesis Mutation Discussion

The majority of your discussion should give MEANING to the results. That is,

the discussion doesn't just restate the results; it explains what the results mean for the

experiment, for the lab, and for genetics and biology in general.

What question did we seek to answer with this lab? You should start there, and then

Borough of Manhattan Bacterial Mutagenesis Mutation Discussion

think about the following points through the lens of that question.

Think about what we discussed before and while doing this lab. Remember that we

examined our plates three times – we looked across dilutions, across treatments, and

then we counted actual colonies. To properly analyze this experiment, you should

consider your data from these perspectives when discussing it.

The discussion should NOT be written as a series of answers to these questions – these

ideas and considerations should be woven throughout a thoughtful,

thorough discussion of the whole experiment. The plates you got are a simulation of an

actual experiment; treat it as such – although you did not do the setup yourself, you can

still discuss the factors that go into performing such an experiment.

Dilutions: What did our serial dilutions tell us? Why are serial dilutions so powerful and

where are they most effective? How did they affect our data and counting? How did they

help us answer our experimental question?

Treatments: What did we learn from our treatments? How did they affect our colony

Borough of Manhattan Bacterial Mutagenesis Mutation Discussion

growth, and therefore our data? Were they as you predicted, or were you surprised by

any results? Can you answer our experimental question? Estimates are fine, killing

curves are always an educated estimation. Borough of Manhattan Bacterial Mutagenesis Mutation Discussion

Colony Data: How did your data turn out? Does the overall shape of your graph make

sense? If it does, what does it tell you about our experimental question? What

conclusions can you draw from it? Are there any outliers? How might those have come

about? Can you still draw conclusions regarding our experimental question? Can you

estimate the UV dose that would result in 1% survival? What does this number tell you?

If the graph does not make sense, in what way? What are some possible sources of

error in this experiment? What might you do differently?

Finally, be sure to connect the bacterial mutagenesis results to genetics and biology in

general.

Borough of Manhattan Bacterial Mutagenesis Mutation Discussion

RUBRIC
Excellent Quality 95-100%	Introduction 45-41 points The background and significance of the problem and a clear statement of the research purpose is provided. The search history is mentioned.		Literature Support 91-84  points The background and significance of the problem and a clear statement of the research purpose is provided. The search history is mentioned.			Methodology 58-53 points Content is well-organized with headings for each slide and bulleted lists to group related material as needed. Use of font, color, graphics, effects, etc. to enhance readability and presentation content is excellent. Length requirements of 10 slides/pages or less is met.
Average Score 50-85%	40-38 points More depth/detail for the background and significance is needed, or the research detail is not clear. No search history information is provided.		83-76  points Review of relevant theoretical literature is evident, but there is little integration of studies into concepts related to problem. Review is partially focused and organized. Supporting and opposing research are included. Summary of information presented is included. Conclusion may not contain a biblical integration.			52-49  points Content is somewhat organized, but no structure is apparent. The use of font, color, graphics, effects, etc. is occasionally detracting to the presentation content. Length requirements may not be met.
Poor Quality 0-45%	37-1 points The background and/or significance are missing. No search history information is provided.		75-1 points Review of relevant theoretical literature is evident, but there is no integration of studies into concepts related to problem. Review is partially focused and organized. Supporting and opposing research are not included in the summary of information presented. Conclusion does not contain a biblical integration.			48-1 points There is no clear or logical organizational structure. No logical sequence is apparent. The use of font, color, graphics, effects etc. is often detracting to the presentation content. Length requirements may not be met
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